Beilstein J. Org. Chem.2014,10, 2348–2360, doi:10.3762/bjoc.10.245
single strands to give such large targets, with only a modest amount of design to ensure minimal off-target annealing. With ideal DNA, plasmid-sized constructs (L-DNA, 1,000–10,000 base pairs) and possibly even ultra-longDNAconstructs (UL-DNA > 10,000 base pairs) might assemble simply by combining the
constructs, including errors in the synthesis of the DNA fragments used for the assembly. These must be mitigated in any effort to look forward, especially in the hope of finding inexpensive automated strategies that assemble inexpensive oligonucleotides into longDNAconstructs (L-DNA) and (possibly
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Graphical Abstract
Figure 1:
Structures explaining why large DNA (L-DNA) constructs cannot easily autonomously self-assemble fro...
Beilstein J. Org. Chem.2014,10, 1826–1833, doi:10.3762/bjoc.10.192
a scalable and integrated infrastructure for the rapid and designed engineering of biology.
Keywords: AEGIS; bioinformatics; DNA self-assembly; longDNAconstructs; software; Introduction
Automated synthesis of single stranded DNA fragments has, perhaps more than any other technology, enabled the
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Graphical Abstract
Figure 1:
Some structures showing possible reasons why large DNA (L-DNA) constructs do not self-assemble from...